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KMID : 0903619880290030159
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Journal of the Korean Society for Horticultural Science
1988 Volume.29 No. 3 p.159 ~ p.170
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Effect of Sodium Chloride and Sodium Sulfate on Growth and Metabolite Content in Shoot Tip Culture of Chinese Cabbage (Brassica campestris L. ssp pekinensis Rupr)
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Abstract
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This study was carried out to select salt tolerance lines during long term subcultures of Chinese cabbage (Braassica campestris L. ssp. pekinensis Rupr) shoot cultures on the media with NaCl and Na₂SO₄, and to investigate the physiological adaptation to salt by analyzing some metabolites of shoots induced during selection. Fresh weight in 0.5% and 1.0% of NaCl and 0.5% of Na₂SO₄ more increased, but shoot number much decreased, than the control. Protein content of shoots increased by NaCl but was not affected by Na2S04 regardless of number of subculture. The starch content of shoot increased in accordance with number of subculture and increase of salt concentration in the media. When treated with 1.0% NaCl or 0.5% Na₂SO₄ the shoot content of a-amino nitrogen decreased in accordance with the number of subculture. Sucrose content decreased with the number of subculture but reducing sugar was not influenced. The content of protein and phenolics decreased by increasing salt concentration. Chlorophyll content decreased in proportion to increasing salts. Selection for salt tolerance did not affect the subsequent growth on media with salts. Salts, especially NaCl, decreased water and osmotic potential of leaf. 0.5% NaCl or Na₂SO₄ delayed the rooting but increased root fresh weight. 1.0% NaCl decreased percentage of rooting and fresh weight.
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